PA5-88983

antibody from Invitrogen Antibodies

Targeting: HFE HFE1, HLA-H

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-88983

Provider

Invitrogen Antibodies

Product name

HFE Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: SSQMWLQLSQ SLKGWDHMFT VDFWTIMENH NHSKESHTLQ VILGCEMQED NSTEGYWKYG YDGQDHLEFC PDTLDWRAAE PRAWPTKLEW ERHKIRARQN RAYLERDCPA QLQQLLELGR GVLDQQVPPL VKVTHHVTSS VTTLRCRALN YYPQNITMKW LKDKQPMDAK EFEPKDVLPN GDGTYQGWIT LAVPPGEEQR YTCQVEHPGL DQPLIVIWEP SPSGTLV; Positive Samples: Mouse liver, Mouse heart; Cellular Location: Cell membrane, Single-pass type I membrane protein

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.66 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using HFE Polyclonal antibody (Product # PA5-88983) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of HFE in extracts of various cell lines using HFE Polyclonal Antibody (Product # PA5-88983) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of MCF-7 cells using HFE Polyclonal antibody (Product # PA5-88983). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of HFE was performed in MCF-7 cells using HFE Polyclonal Antibody (Product # PA5-88983).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of HFE was performed in MCF-7 cells using HFE Polyclonal Antibody (Product # PA5-88983).