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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PA5-37919 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SPON2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with mouse, rat and dog based on sequence homology.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of Spondin-2/Mindin in A549 lysate was performed using 35 µg protein in RIPA buffer. Lysates were probed with a Spondin-2/Mindin polyclonal antibody (Product # PA5-37919) at a dilution of 1 µg/mL and incubated for 1 hour, followed by chemiluminescent detection.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of SPON2 in Human Tonsil using a SPON2 monoclonal antibody (Product #PA5-37919) at 5 µg/mL. The Human Tonsil tissue section was paraffin embeded and detected using steamed antigen retrieval with citrate buffer pH 6, AP-staining.
