PA5-39270

antibody from Invitrogen Antibodies

Targeting: NDUFA9 CI-39k, COQ11, NDUFS2L, SDR22E1

Antibody data

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Validation data

NDUFA9 protein structure - PA5-39270 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of NDUFA9 in extracts from HeLa cells using a NDUFA9 polyclonal antibody (Product # PA5-39270).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: KD of NDUFA9 was achieved by transfecting Hep G2 cells with NDUFA9 specific siRNAs (Silencer® select Product # s39346, s9347). Western blot analysis (Fig. a) was performed using membrane enriched cell extracts from the NDUFA9 KD cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed NDUFA9 Polyclonal Antibody (Product # PA5-39270, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to NDUFA9.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-NDUFA9 Polyclonal Antibody (Product # PA5-39270) and a 36 kDa band corresponding to NDUFA9 was observed across cell lines and tissues tested. Membrane enriched cell extracts (30 µg lysate) of HeLa (Lane 1), Caco-2 (Lane 2), SW480 (Lane 3), Hep G2 (Lane 4), Jurkat (Lane 5), K-562 (Lane 6), MCF-7 (Lane 7), Mouse Liver (Lane 8), Rat Liver (Lane 9), Mouse Brain (Lane 10) and Rat Brain (Lane 11) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence using Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).