PA5-30030

antibody from Invitrogen Antibodies

Targeting: FGL1 HFREP-1, HPS

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30030

Provider

Invitrogen Antibodies

Product name

FGL1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HepG2, mouse liver.

Concentration

0.76 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of FGL1 using 50 µg of mouse liver lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a FGL1 polyclonal antibody (Product # PA5-30030) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of FGL1 using 30 µg of HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a FGL1 polyclonal antibody (Product # PA5-30030) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using FGL1 Polyclonal Antibody (Product # PA5-30030). Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with FGL1 Polyclonal Antibody (Product # PA5-30030) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FGL1 Polyclonal Antibody detects FGL1 protein at cytosol on gastric carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. FGL1 Polyclonal Antibody (Product # PA5-30030) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.