TA323495

antibody from OriGene

Targeting: ATP5PF ATP5, ATP5A, ATP5J, ATPM, CF6

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA323495

Provider

OriGene

Product name

Anti-ATP5J rabbit polyclonal antibody

Antibody type

Polyclonal

Description

Anti-ATP5J rabbit polyclonal antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

ATP5PF

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µl

Concentration

0.6mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted band size: 13 kDa . Positive control: Human fetal brain tissue and 293T cell, mouse intestinum tenue tissue and lovo cell lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lane1: Human fetal brain tissue lysate Lane2: 293T cell lysate Lane3: Mouse intestinum tenue tissue lysate Lane4: Lovo cell lysate Lysates: 40 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted cell location: Cytoplasm. Positive control: Human colon cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer using ATP5J antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification: x 200)

Validation comment

IHC