Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB267-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal NT-3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human NT-3 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human (rh) BDNF, rhCNTF, rhGDNF, rh beta-NGF, and rhNT-4 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by NT-3 and Neutralization by Human NT-3 Antibody. Recombinant Human NT-3 (Catalog # 267-N3) stimulates proliferation in BaF-TrKB-BD mouse pro-B cell line transfected with TrkB in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human NT-3 (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human NT-3 Monoclonal Antibody (Catalog # MAB267). The ND50 is typically 0.1-0.5 µg/mL.