Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PA5-88410 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EFHA1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: AWHHSRVSVA ARDGSFTVSA QKNVEHGIIY IGKPSLRKQR FMQFSSLEHE GEYYMTPRDF LFSVMFEQME RKTSVKKLTK KDIEDTLSGI QTAGCGSTFF R; Positive Samples: 293T, Mouse skeletal muscle, Rat skeletal muscle; Cellular Location: Mitochondrion intermembrane space
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.05 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of extracts of various cell lines, using MICU2 Polyclonal antibody (Product # PA5-88410) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 10s.