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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-21423 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RanBP16 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLaS3.
- Concentration
- 0.93 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RanBP16 using 30 µg of A) H1299 and B) HeLa S3 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a RanBP16 polyclonal antibody (Product # PA5-21423) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RanBP16 Polyclonal Antibody (Product # PA5-21423). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RanBP16 Polyclonal Antibody (Product # PA5-21423) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RanBP16 Polyclonal Antibody (Product # PA5-21423). Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RanBP16 Polyclonal Antibody (Product # PA5-21423) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lung SCC, using RanBP16 (Product # PA5-21423) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
