Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- AF6106 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal MCAM/CD146 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse MCAM/CD146 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant rat MCAM is observed and approximately 10% cross-reactivity with recombinant human MCAM is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human and Mouse MCAM/CD146 by Western Blot. Western blot shows lysates of Bowes human melanoma cell line and mouse embryo tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse MCAM/CD146 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6106) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MCAM/CD146 at approximately 117 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse MCAM/CD146 by Simple WesternTM. Simple Western lane view shows lysates of mouse embryo tissue (15 d.p.c.), loaded at 0.2 mg/mL. A specific band was detected for MCAM/CD146 at approximately 138 kDa (as indicated) using 10 µg/mL of Sheep Anti-Mouse MCAM/CD146 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6106) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.