FAB932P-100

antibody from R&D Systems

Targeting: MCAM CD146, HEMCAM, MelCAM, METCAM, MUC18

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

FAB932P-100

Provider

R&D Systems

Product name

Human MCAM/CD146 PE-conjugated Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human MCAM in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) ALCAM, rhBCAM, rhEpCAM, rhNCAM-L1, recombinant mouse (rm) OCAM, or rmMAdCAM-1.

Reactivity

Human

Host

Mouse

Antigen sequence

AAA20824


Isotype

IgG

Antibody clone number

128018

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

References

Submitted references

Phenotyping of human melanoma cells reveals a unique composition of receptor targets and a subpopulation co-expressing ErbB4, EPO-R and NGF-R.

Mirkina I, Hadzijusufovic E, Krepler C, Mikula M, Mechtcheriakova D, Strommer S, Stella A, Jensen-Jarolim E, Höller C, Wacheck V, Pehamberger H, Valent P
PloS one 2014;9(1):e84417

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Human fetal and adult bone marrow-derived mesenchymal stem cells use different signaling pathways for the initiation of chondrogenesis.

Brady K, Dickinson SC, Guillot PV, Polak J, Blom AW, Kafienah W, Hollander AP
Stem cells and development 2014 Mar 1;23(5):541-54

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EBNA3B-deficient EBV promotes B cell lymphomagenesis in humanized mice and is found in human tumors.

White RE, Rämer PC, Naresh KN, Meixlsperger S, Pinaud L, Rooney C, Savoldo B, Coutinho R, Bödör C, Gribben J, Ibrahim HA, Bower M, Nourse JP, Gandhi MK, Middeldorp J, Cader FZ, Murray P, Münz C, Allday MJ
The Journal of clinical investigation 2012 Apr;122(4):1487-502

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Human mesenchymal stromal cells express CD14 cross-reactive epitopes.

Pilz GA, Braun J, Ulrich C, Felka T, Warstat K, Ruh M, Schewe B, Abele H, Larbi A, Aicher WK
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2011 Aug;79(8):635-45

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of MCAM/CD146 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human MCAM/CD146 PE-conjugated Monoclonal Antibody (Catalog # FAB932P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.