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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- NBP2-59697 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal CD74 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Detects approx 80, 55kDa. No cross-reactivity against Cav Beta4.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD74 Antibody (3D7) [NBP2-59697] - Analysis of Human Lymphoblastoid cell line (Raji) showing detection of 33-35 kDa CD74 protein using Mouse Anti-CD74 Monoclonal Antibody, Clone 3D7 . Lane 1: Molecular Weight Ladder (MW). Lane 2: Raji cell lysate. Load: 15 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 33-35 kDa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD74 Antibody (3D7) [NBP2-59697] - Analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone 3D7 . Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:100 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Cells were subject to heat shock at 42C for 2 hours and transferred to CO2 incubator at 37C for 1 hour before trypsinization.
