Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 11-0748-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD74 Monoclonal Antibody (5-329), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 5-329 monoclonal antibody recognizes human CD74, also known as the MHC Class II-associated invariant chain. A 33-43 kDa nonpolymorphic type II membrane protein, CD74 is highly expressed on B cells and subsets of activated T cells, Langerhans cells, dendritic cells, and epithelial cells. Monocytes and macrophages also express CD74, but at lower levels. CD74 acts as a chaperone of MHC Class II (HLA-DR) proteins, promoting their translocation from the ER to endocytic compartments during antigen presentation. Activation of NFkappaB and ERK has been shown following CD74 interaction with CD44 and binding to the proinflammatory cytokine macrophage migration-inhibitory factor (MIF). CD74 also plays a role in the maturation of follicular B-cells and accumulation of marginal zone B-cells. CD74 has also been demonstrated to interact with CXCR2 and CXCR4. Applications Reported: This 5-329 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 5-329 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- 5-329
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Structural Remodeling of the Human Colonic Mesenchyme in Inflammatory Bowel Disease.
FOXP1 suppresses immune response signatures and MHC class II expression in activated B-cell-like diffuse large B-cell lymphomas.
A functional heteromeric MIF receptor formed by CD74 and CXCR4.
CD74 is a member of the regulated intramembrane proteolysis-processed protein family.
Invariant chain targets HLA class II molecules to acidic endosomes containing internalized influenza virus.
Kinchen J, Chen HH, Parikh K, Antanaviciute A, Jagielowicz M, Fawkner-Corbett D, Ashley N, Cubitt L, Mellado-Gomez E, Attar M, Sharma E, Wills Q, Bowden R, Richter FC, Ahern D, Puri KD, Henault J, Gervais F, Koohy H, Simmons A
Cell 2018 Oct 4;175(2):372-386.e17
Cell 2018 Oct 4;175(2):372-386.e17
FOXP1 suppresses immune response signatures and MHC class II expression in activated B-cell-like diffuse large B-cell lymphomas.
Brown PJ, Wong KK, Felce SL, Lyne L, Spearman H, Soilleux EJ, Pedersen LM, Møller MB, Green TM, Gascoyne DM, Banham AH
Leukemia 2016 Mar;30(3):605-16
Leukemia 2016 Mar;30(3):605-16
A functional heteromeric MIF receptor formed by CD74 and CXCR4.
Schwartz V, Lue H, Kraemer S, Korbiel J, Krohn R, Ohl K, Bucala R, Weber C, Bernhagen J
FEBS letters 2009 Sep 3;583(17):2749-57
FEBS letters 2009 Sep 3;583(17):2749-57
CD74 is a member of the regulated intramembrane proteolysis-processed protein family.
Becker-Herman S, Arie G, Medvedovsky H, Kerem A, Shachar I
Molecular biology of the cell 2005 Nov;16(11):5061-9
Molecular biology of the cell 2005 Nov;16(11):5061-9
Invariant chain targets HLA class II molecules to acidic endosomes containing internalized influenza virus.
Lamb CA, Yewdell JW, Bennink JR, Cresswell P
Proceedings of the National Academy of Sciences of the United States of America 1991 Jul 15;88(14):5998-6002
Proceedings of the National Academy of Sciences of the United States of America 1991 Jul 15;88(14):5998-6002
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD19 eFluor® 450 (Product # 48-0199-42) and Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human CD74 FITC (right). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Green dye