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Other assayAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Other assay [1]
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Validation data
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- Product number
- PA5-46934 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FGF17 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human (rh) FGF-8b and recombinant mouse FGF-8c is observed and less than 2% cross-reactivity with rhFGF acidic, rhFGF basic, rhFGF-4, rhFGF-5, rhFGF-6, rhFGF-7, rhFGF-10, and rhFGF-18 is observed.
- Concentration
- 0.2 mg/mL
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Neutralization of FGF17 in NR6R-3T3 mouse fibroblast cell line. Samples were incubated in FGF17 polyclonal antibody (Product # PA5-46934). Recombinant Human FGF-17 stimulates proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF-17 (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF-17 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 6-24 µg/mL in the presence of heparin (1 µg/mL).
