IC13991P

antibody from Novus Biologicals

Targeting: IL25 IL-17E, IL-25, IL17E

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC13991P

Provider

Novus Biologicals

Product name

Rat Monoclonal IL-17E/IL-25 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects mouse IL-17E/IL-25 in ELISAs. In ELISAs, this antibody does not cross-react with recombinant human IL-17E, recombinant mouse IL-17, IL-17B, C, D, or F.

Reactivity

Mouse

Host

Rat

Isotype

IgG

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of IL-17E/IL-25 in Tramp-C1 Mouse Cell Line by Flow Cytometry. Tramp-C1 mouse prostate cancer cell line was stained with Rat Anti-Mouse IL-17E/IL-25 PE-conjugated Monoclonal Antibody (Catalog # IC13991P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.