TA505665

antibody from OriGene

Targeting: HRAS HRAS1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA505665

Provider

OriGene

Proper citation

OriGene Cat#TA505665, RRID:AB_2623266

Product name

HRAS mouse monoclonal antibody, clone OTI1F2 (formerly 1F2)

Antibody type

Monoclonal

Description

HRAS mouse monoclonal antibody, clone OTI1F2 (formerly 1F2)

Host

Mouse

Conjugate

Unconjugated

Epitope

HRAS

Isotype

IgG

Antibody clone number

OTI1F2

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HRAS (RC216409, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HRAS.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HRAS monoclonal antibody at 1:200 (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-HRAS mouse monoclonal antibody (TA505665) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HRAS(RC216409).

Validation comment

IF