MAB2417

antibody from Novus Biologicals

Targeting: OLIG1 BHLHB6, bHLHe21

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB2417

Provider

Novus Biologicals

Product name

Mouse Monoclonal Olig1 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human and mouse Olig1. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) Olig2 or rhOlig3.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Shortening the Half-Life of Cas9 Maintains Its Gene Editing Ability and Reduces Neuronal Toxicity.

Yang S, Li S, Li XJ
Cell reports 2018 Dec 4;25(10):2653-2659.e3

---

Demyelination causes adult CNS progenitors to revert to an immature state and express immune cues that support their migration.

Moyon S, Dubessy AL, Aigrot MS, Trotter M, Huang JK, Dauphinot L, Potier MC, Kerninon C, Melik Parsadaniantz S, Franklin RJ, Lubetzki C
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Jan 7;35(1):4-20

---

Reduced Morg1 expression in ischemic human brain.

Haase D, Keiner S, Mawrin C, Wolf G
Neuroscience letters 2009 May 8;455(1):46-50

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Olig1 by Western Blot. Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line and SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human Olig1 Monoclonal Antibody (Catalog # MAB2417) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Olig1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Olig1 in Human Glioma. Olig1 was detected in immersion fixed paraffin-embedded sections of human glioma using 8 µg/mL Human Olig1 Monoclonal Antibody (Catalog # MAB2417) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.