Explore
Validate
Learn
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Flow cytometry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- FAB2319P - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal LAG-3 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human LAG-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse LAG-3 is observed.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Submitted references Compensatory upregulation of PD-1, LAG-3, and CTLA-4 limits the efficacy of single-agent checkpoint blockade in metastatic ovarian cancer.
Interleukin-10-producing LAG3(+) regulatory T cells are associated with disease activity and abatacept treatment in rheumatoid arthritis.
CD62L+ NKT cells have prolonged persistence and antitumor activity in vivo.
Immunomodulation Induced by Stem Cell Mobilization and Harvesting in Healthy Donors: Increased Systemic Osteopontin Levels after Treatment with Granulocyte Colony-Stimulating Factor.
TGF-β3-expressing CD4+CD25(-)LAG3+ regulatory T cells control humoral immune responses.
Cytomegalovirus-Induced Expression of CD244 after Liver Transplantation Is Associated with CD8+ T Cell Hyporesponsiveness to Alloantigen.
Trafficking of LAG-3 to the surface on activated T cells via its cytoplasmic domain and protein kinase C signaling.
Human autoimmunity after lymphocyte depletion is caused by homeostatic T-cell proliferation.
Huang RY, Francois A, McGray AR, Miliotto A, Odunsi K
Oncoimmunology 2017;6(1):e1249561
Oncoimmunology 2017;6(1):e1249561
Interleukin-10-producing LAG3(+) regulatory T cells are associated with disease activity and abatacept treatment in rheumatoid arthritis.
Nakachi S, Sumitomo S, Tsuchida Y, Tsuchiya H, Kono M, Kato R, Sakurai K, Hanata N, Nagafuchi Y, Tateishi S, Kanda H, Okamura T, Yamamoto K, Fujio K
Arthritis research & therapy 2017 May 16;19(1):97
Arthritis research & therapy 2017 May 16;19(1):97
CD62L+ NKT cells have prolonged persistence and antitumor activity in vivo.
Tian G, Courtney AN, Jena B, Heczey A, Liu D, Marinova E, Guo L, Xu X, Torikai H, Mo Q, Dotti G, Cooper LJ, Metelitsa LS
The Journal of clinical investigation 2016 Jun 1;126(6):2341-55
The Journal of clinical investigation 2016 Jun 1;126(6):2341-55
Immunomodulation Induced by Stem Cell Mobilization and Harvesting in Healthy Donors: Increased Systemic Osteopontin Levels after Treatment with Granulocyte Colony-Stimulating Factor.
Melve GK, Ersvaer E, Akkök ÇA, Ahmed AB, Kristoffersen EK, Hervig T, Bruserud Ø
International journal of molecular sciences 2016 Jul 19;17(7)
International journal of molecular sciences 2016 Jul 19;17(7)
TGF-β3-expressing CD4+CD25(-)LAG3+ regulatory T cells control humoral immune responses.
Okamura T, Sumitomo S, Morita K, Iwasaki Y, Inoue M, Nakachi S, Komai T, Shoda H, Miyazaki J, Fujio K, Yamamoto K
Nature communications 2015 Feb 19;6:6329
Nature communications 2015 Feb 19;6:6329
Cytomegalovirus-Induced Expression of CD244 after Liver Transplantation Is Associated with CD8+ T Cell Hyporesponsiveness to Alloantigen.
de Mare-Bredemeijer EL, Shi XL, Mancham S, van Gent R, van der Heide-Mulder M, de Boer R, Heemskerk MH, de Jonge J, van der Laan LJ, Metselaar HJ, Kwekkeboom J
Journal of immunology (Baltimore, Md. : 1950) 2015 Aug 15;195(4):1838-48
Journal of immunology (Baltimore, Md. : 1950) 2015 Aug 15;195(4):1838-48
Trafficking of LAG-3 to the surface on activated T cells via its cytoplasmic domain and protein kinase C signaling.
Bae J, Lee SJ, Park CG, Lee YS, Chun T
Journal of immunology (Baltimore, Md. : 1950) 2014 Sep 15;193(6):3101-12
Journal of immunology (Baltimore, Md. : 1950) 2014 Sep 15;193(6):3101-12
Human autoimmunity after lymphocyte depletion is caused by homeostatic T-cell proliferation.
Jones JL, Thompson SA, Loh P, Davies JL, Tuohy OC, Curry AJ, Azzopardi L, Hill-Cawthorne G, Fahey MT, Compston A, Coles AJ
Proceedings of the National Academy of Sciences of the United States of America 2013 Dec 10;110(50):20200-5
Proceedings of the National Academy of Sciences of the United States of America 2013 Dec 10;110(50):20200-5
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of LAG-3 in Human PBMCs by Flow Cytometry. CD3+ PHA-treated peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Goat Anti-Human LAG-3 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB2319P) or (B) Normal Goat IgG Phycoerythrin Control (Catalog # IC108P). View our protocol for Staining Membrane-associated Proteins.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of LAG-3 in HEK293 Human Cell Line Transfected with Human LAG-3 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human LAG-3 or (B) irrelelvant transfectants and eGFP was stained with Goat Anti-Human LAG-3 Phycoerythrin-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB2319P). Quadrant markers were set based on control antibody staining (Catalog # IC108P, data not shown). View our protocol for Staining Membrane-associated Proteins.
