369205

antibody from BioLegend

Targeting: LAG3 CD223

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

369205

Provider

BioLegend

Proper citation

BioLegend Cat#369205, RRID:AB_2632655

Product name

PE anti-human CD223 (LAG-3)

Antibody type

Monoclonal

Description

CD223, also known as LAG-3, is a 70 kD type I transmembrane glycoprotein that is involved in T-cell signaling. Similar to CD4, CD223 binds MHC class II, but with a higher affinity. CD223 negatively regulates T-cell activation. It is expressed by activated T-cells and natural killer cells (NKs), as well as regulatory T-cells. It is transiently expressed on the surface of activated T-cells in acute conditions but high expression is maintained under tolerizing conditions. CD223 deficiency results in reduced tumor growth. CD223 and PD-1 can act in synergy and reverse exhausted phenotypes, improve tumor rejection, and control viral load.

Reactivity

Human

Host

Mouse

Conjugate

Yellow dye

Antibody clone number

7H2C65

Vial size

25 tests

Storage

2°C-8°C

Handling

Ambient RT

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

CD3/CD28/IL-2 stimulated (three days) peripheral blood monocular cells (PBMCs) were stained with CD8 Alexa Fluor® 647 and CD223 (clone 7H2C65) PE (top) or mouse IgG1, κ PE isotype control (bottom).

Conjugate

Yellow dye