Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
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Validation data
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- Product number
- PA5-47358 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LOXL2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, approximately 1% cross-reactivity with recombinant human LOXL3 is observed.
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates ofU-87 MG human glioblastoma/astrocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Lysyl Oxidase Homolog 2/LOXL2 at approximately 109 kDa (as indicated) using 10 µg/mL of Goat Anti-human Lysyl Oxidase Homolog 2/LOXL2 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47358) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody. This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates ofU-87 MG human glioblastoma/astrocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Lysyl Oxidase Homolog 2/LOXL2 at approximately 109 kDa (as indicated) using 10 µg/mL of Goat Anti-human Lysyl Oxidase Homolog 2/LOXL2 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47358) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody. This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LOXL2 in HEC‚1‚B human endometrial adenocarcinoma cell line and U‚87 MG human glioblastoma/astrocytoma cell line. Samples were incubated in LOXL2 polyclonal antibody (Product # PA5-47358) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for Lysyl Oxidase Homolog 2/LOXL2 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LOXL2 in 0.2 mg/mL lysates of U‚87 MG human glioblastoma/astrocytoma cell line. Samples were incubated in LOXL2 polyclonal antibody (Product # PA5-47358) using a dilution of 10 µg/mL followed by HRP-conjugated Anti-Goat IgG at a dilution of 0.0763888888888889. A specific band was detected for Lysyl Oxidase Homolog 2/LOXL2 at approximately 109 kDa (as indicated). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.