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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [9]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-85210 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LOXL2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution.
- Concentration
- 0.7 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LOXL2 in various whole cell extracts using LOXL2 polyclonal antibody (Product # PA5-85210) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LOXL2 in mouse tissue extract using LOXL2 polyclonal antibody (Product # PA5-85210) using 50 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LOXL2 in wild-type (WT) and LOXL2 knockout (KO) HeLa cell extracts using LOXL2 polyclonal antibody (Product # PA5-85210) using 30 µg of sample at a dilution of 1:500. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of LOXL2 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a LOXL2 Polyclonal Antibody (Product # PA5-85210) at a dilution of 1:1,000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LOXL2 in various whole cell extracts using LOXL2 polyclonal antibody (Product # PA5-85210) using 30 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LOXL2 in non-transfected (–) and transfected (+) 293T whole cell extracts using LOXL2 polyclonal antibody (Product # PA5-85210) using 30 µg of sample at a dilution of 1:5000. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of LOXL2 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a LOXL2 Polyclonal Antibody (Product # PA5-85210) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of LOXL2 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a LOXL2 Polyclonal Antibody (Product # PA5-85210) at a dilution of 1:1,000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of LOXL2 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a LOXL2 Polyclonal Antibody (Product # PA5-85210) at a dilution of 1:5,000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of LOXL2 in A431 cells using LOXL2 polyclonal antibody (Product # PA5-85210) at a dilution of 1:500. Sample was then incubated with Hoechst secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of LOXL2 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: LOXL2 Polyclonal Antibody (Product # PA5-85210) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of LOXL2 was performed in paraffin-embedded human ovarian cancer tissue using LOXL2 Polyclonal Antibody (Product # PA5-85210) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of LOXL2 was performed in paraffin-embedded mouse ovary tissue using LOXL2 Polyclonal Antibody (Product # PA5-85210) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
