Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-56327 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMYD4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: ACQTEAHRMA AGPRWEAFCC NSCGAPMQGD DVLRCGSRSC AESAVSRDHL VSRLQDLQQQ VRVAQKLLRD GELERAVQRL SGCQR
- Concentration
- 0.1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMYD4 in control (vector only transfected HEK293T lysate) and SMYD4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a SMYD4 Polyclonal Antibody (Product # PA5-56327).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of SMYD4 in human cell line A-431 shows positivity in the Golgi apparatus, vesicles & nucleus but excluded from the nucleoli. Samples were probed using a SMYD4 Polyclonal Antibody (Product # PA5-56327).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SMYD4 in human ovary using a SMYD4 Polyclonal Antibody (Product # PA5-56327) shows cytoplasmic positivity in follicle cells.