MAB2859R-100

antibody from R&D Systems

Targeting: SIGLEC6 CD327, CD33L, CD33L1, OB-BP1, SIGLEC-6

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB2859R-100

Provider

R&D Systems

Product name

Human Siglec-6/CD327 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from cell culture supernatant. Detects human Siglec-6/CD327 in direct ELISAs.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

O43699


Isotype

IgG

Antibody clone number

767329R

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Siglec-6/CD327 in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human Siglec-6/CD327 Monoclonal Antibody (Catalog # MAB2859R, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Siglec-6/CD327 in Human PBMC lymphocytes by Flow Cytometry. Human peripheral blood mononuclear cell (PBMC) lymphocytes were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human Siglec-6/CD327 Monoclonal Antibody (Catalog # MAB2859R) or (B) Mouse IgG_2A Isotype Control (Catalog # MAB003) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.