702669

antibody from Invitrogen Antibodies

Targeting: ELP3 FLJ10422, KAT9

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

702669

Provider

Invitrogen Antibodies

Product name

ELP3 Recombinant Rabbit Monoclonal Antibody (8H8L24)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with Monkey, Pig, Horse, Bovine.

Antibody clone number

8H8L24

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of ELP3 was achieved by transfecting SH-SY5Y cells with ELP3 specific siRNA (Silencer® select Product # s30262 and Product # s30264). Western blot analysis (Fig a) was performed using whole cell extracts from the SH-SY5Y knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blots were probed with Anti-ELP3 Recombinant Rabbit Monoclonal Antibody (Product # 702669, 1:200 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution). Densitometric analysis of this Western blot is shown in histogram (Fig b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to ELP3.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of IMR-32 (Lane 1), U-87 MG (Lane 2), HeLa (Lane 3), A549 (Lane 4), Neuro-2a (Lane 5), HT-29 (Lane 6), MDA-MB-231 (Lane 7) and tissue extract (30 µg lysate) of Mouse Brain (Lane 8). The blot was probed with Anti-ELP3 Recombinant Rabbit Monoclonal Antibody (Product # 702669, 1:200 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution). A ~62 kDa band corresponding to ELP3 was observed across the cell lines tested.