63-9488-42

antibody from Invitrogen Antibodies

Targeting: KLRG1 2F1, CLEC15A, MAFA, MAFA-L

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

63-9488-42

Provider

Invitrogen Antibodies

Product name

KLRG1 Monoclonal Antibody (13F12F2), Super Bright™ 600, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: This 13F12F2 monoclonal antibody reacts with human killer cell lectin-like receptor subfamily G, member 1 (KLRG1), a type II transmembrane inhibitor receptor of the C-type lectin superfamily that contains an ITIM domain. This inhibitory receptor is expressed on subsets of gamma-delta T cells, NK (CD56dim), CD8+ and CD4+ T Cells. KLRG1 is expressed primarily by cells with an effector/memory phenotype that are short-lived, but capable of immediate effector cell function. Cadherin-E, -N, and -R are ligands for KLRG1. Cadherin/KLRG1 interaction inhibits cytolytic activity and proliferation. The percentage of KLRG1 positive cells can vary considerably, depending on antigen experience.

Antibody clone number

13F12F2

Concentration

5 µL/Test

References

Submitted references

Isolation of tumor-resident CD8(+) T cells from human lung tumors.

Corgnac S, Lecluse Y, Mami-Chouaib F
STAR protocols 2021 Mar 19;2(1):100267

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Normal human peripheral blood cells cells were stained with CD28 Monoclonal Antibody, FITC (Product # 11-0289-42) and Mouse IgG2a kappa Isotype Control, Super Bright 600 (Product # 63-4724-82) (left) or KLRG1 Monoclonal Antibody, Super Bright 600 (right). Cells in the lymphocyte gate were used for analysis.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Normal human peripheral blood cells cells were stained with CD56 Monoclonal Antibody, PerCP-eFluor 710 (Product # 46-0567-42) and Mouse IgG2a kappa Isotype Control, Super Bright 600 (Product # 63-4724-82) (left) or KLRG1 Monoclonal Antibody, Super Bright 600 (right). Cells in the lymphocyte gate were used for analysis.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Normal human peripheral blood cells were stained with CD8a Monoclonal Antibody, APC (Product # 17-0087-42) and Mouse IgG2a kappa Isotype Control, Super Bright 600 (Product # 63-4724-82) (left) or KLRG1 Monoclonal Antibody, Super Bright 600 (right). Cells in the lymphocyte gate were used for analysis.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 Gating strategy for sorting CD8 + CD103 + and CD8 + KLRG1 + cells by FACS