GTX630983

antibody from GeneTex

Targeting: FIS1 CGI-135, H_NH0132A01.6, TTC11

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX630983

Provider

GeneTex

Product name

FIS1 antibody [GT4211]

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Western blot

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody [GT4211] (GTX630983) diluted at 1:500.

KD Reagent Type

siRNA

Downregulation

>50%

KD Reagent Prod page

KD Reagent Prod page

KD Reagent Provider

shRNA reagents was provided courtesy of cooperative labs.

KD Reagent Prod no

Proprietary.

Antibody Lot Number

41904

Show more

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

FIS1 antibody detects FIS1 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX630983) diluted by 1:1000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody [GT4211] (GTX630983) diluted at 1:500.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

FIS1 antibody detects FIS1 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.Green: FIS1 protein stained by FIS1 antibody (GTX630983) diluted at 1:1000.Red: MitoTrackerR Red CMXRos, a mitochondria tracker.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

FIS1 antibody [GT4211] detects FIS1 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at room temperature.Green: FIS1 protein stained by FIS1 antibody [GT4211] (GTX630983) diluted at 1:100.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.