Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Immunohistochemistry [2]
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Validation data
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- Product number
- MAB1320 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal Endoglin/CD105 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse Endoglin in direct ELISAs and Western blots. In Western blots, this antibody shows 100% cross-reactivity with recombinant human Endoglin.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Volume expansion and TRPV4 activation regulate stem cell fate in three-dimensional microenvironments.
Bistable Epigenetic States Explain Age-Dependent Decline in Mesenchymal Stem Cell Heterogeneity.
Gli1+ Mesenchymal Stromal Cells Are a Key Driver of Bone Marrow Fibrosis and an Important Cellular Therapeutic Target.
Mesenchymal stem cells originating from ES cells show high telomerase activity and therapeutic benefits.
PIR-B-deficient mice are susceptible to Salmonella infection.
PIR-B-deficient mice are susceptible to Salmonella infection.
Lee HP, Stowers R, Chaudhuri O
Nature communications 2019 Jan 31;10(1):529
Nature communications 2019 Jan 31;10(1):529
Bistable Epigenetic States Explain Age-Dependent Decline in Mesenchymal Stem Cell Heterogeneity.
Hamidouche Z, Rother K, Przybilla J, Krinner A, Clay D, Hopp L, Fabian C, Stolzing A, Binder H, Charbord P, Galle J
Stem cells (Dayton, Ohio) 2017 Mar;35(3):694-704
Stem cells (Dayton, Ohio) 2017 Mar;35(3):694-704
Gli1+ Mesenchymal Stromal Cells Are a Key Driver of Bone Marrow Fibrosis and an Important Cellular Therapeutic Target.
Schneider RK, Mullally A, Dugourd A, Peisker F, Hoogenboezem R, Van Strien PMH, Bindels EM, Heckl D, Büsche G, Fleck D, Müller-Newen G, Wongboonsin J, Ventura Ferreira M, Puelles VG, Saez-Rodriguez J, Ebert BL, Humphreys BD, Kramann R
Cell stem cell 2017 Jun 1;20(6):785-800.e8
Cell stem cell 2017 Jun 1;20(6):785-800.e8
Mesenchymal stem cells originating from ES cells show high telomerase activity and therapeutic benefits.
Ninagawa N, Murakami R, Isobe E, Tanaka Y, Nakagawa H, Torihashi S
Differentiation; research in biological diversity 2011 Oct;82(3):153-64
Differentiation; research in biological diversity 2011 Oct;82(3):153-64
PIR-B-deficient mice are susceptible to Salmonella infection.
Torii I, Oka S, Hotomi M, Benjamin WH Jr, Takai T, Kearney JF, Briles DE, Kubagawa H
Journal of immunology (Baltimore, Md. : 1950) 2008 Sep 15;181(6):4229-39
Journal of immunology (Baltimore, Md. : 1950) 2008 Sep 15;181(6):4229-39
PIR-B-deficient mice are susceptible to Salmonella infection.
Torii I, Oka S, Hotomi M, Benjamin WH Jr, Takai T, Kearney JF, Briles DE, Kubagawa H
Journal of immunology (Baltimore, Md. : 1950) 2008 Sep 15;181(6):4229-39
Journal of immunology (Baltimore, Md. : 1950) 2008 Sep 15;181(6):4229-39
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Endoglin/CD105 in Mouse Embryo. Endoglin/CD105 was detected in immersion fixed frozen sections of mouse embryo (13.5 d.p.c.) using Rat Anti-Mouse Endoglin/CD105 Monoclonal Antibody (Catalog # MAB1320) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to endothelial cells of the developing hindlimb. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. Image depicts staining at 10X magnification.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Endoglin/CD105 in Mouse Embryo. Endoglin/CD105 was detected in immersion fixed frozen sections of mouse embryo (13.5 d.p.c.) using Rat Anti-Mouse Endoglin/CD105 Monoclonal Antibody (Catalog # MAB1320) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to endothelial cells of the developing hindlimb. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. Image depicts staining at 40X magnification.