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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- 353804 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#353804, RRID:AB_10965546
- Product name
- PE anti-human CLEC9A (DNGR1)
- Antibody type
- Monoclonal
- Antigen
- CLEC9A
- Description
- CLECA, also known as DNGR1, is a type II transmembrane protein consisting of a single extracellular C-type lectin domain (CTLD) that is connected to the transmembrane domain by a stalk region, and an intracellular cytoplasmic tail with immunoreceptor tyrosine-based activation motif (ITAM). In humans, CLEC9A is strictly expressed on BDCA3-positive conventional dendritic cells (cDC) and CD16- monocytes. CLEC9A recognizes a ubiquitous preformed acid-labile protein associated ligand(s) that is exposed on necrotic cells. CLEC9A ligation is necessary for efficient cross-presentation of dead-cell-associated antigen by recruitment and activation of the tyrosine kinase Syk.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Vial size
- 100 tests
- Storage
- The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- Human peripheral blood mononuclear cells were stained with CD3 PerCP, HLA-DR Alexa Fluor® 488, CD141 APC, and CLEC9a (clone 8F9) PE (top) or mouse IgG2a, κ PE isotype control (bottom). Data was analyzed with CD3-negative and HLA-DR-positive cell population.
- Conjugate
- Yellow dye
