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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [5]
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- Product number
- MA5-27589 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HCN1 Monoclonal Antibody (S70-28)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- S70-28
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HCN1 in rat brain membrane lysate with 15 µg of sample. The sample was blocked with 1.5% BSA, incubated with HCN1 monoclonal antibody (Product # MA5-27589) using a dilution of 1:1000 (2 hours at 4°C), followed by Sheep Anti-Mouse HRP (1 hour at RT).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-HCN1 Monoclonal Antibody (S70-28) (Product # MA5-27589) and a 110 kDa band corresponding to Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 was observed only in Mouse Brain, Rat Brain, Mouse Cerebellum and Rat cerebellum but not in any other tissues tested like Mouse Heart, Rat Heart and Mouse Skeletal Muscle. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Cerebellum (Lane 3), Rat Cerebellum (Lane 4), Mouse Heart (Lane 5), Rat Heart (Lane 6) and Mouse Skeletal Muscle (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Uncharacterized band of ~40 kDa was also observed in Mouse Heart and Rat Heart.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HCN1 in human frozen brain section. Sample was fixed with 10% formalin (12-24 hours at RT), incubated with HCN1 monoclonal antibody (Product # MA5-27589) using a dilution of 1:1000 (1 hour at RT), and followed by Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) and Mayer Hematoxylin (purple/blue) (30 minutes at RT) at a dilution of 250-500 µl.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HCN1 in mouse cerebellum. Samples were incubated with HCN1 monoclonal antibody (Product # MA5-27589) using a dilution of 1:1000 (1 hour at RT) and followed by Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) and Mayer Hematoxylin (purple/blue) at a dilution of 250-500 µl.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HCN1 in human frozen brain section. Sample was fixed with 10% formalin (12-24 hours at RT), incubated with HCN1 monoclonal antibody (Product # MA5-27589) using a dilution of 1:1000 (1 hour at RT), and followed by Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) and Mayer Hematoxylin (purple/blue) (30 minutes at RT) at a dilution of 250-500 µl.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HCN1 in human frozen brain section. Sample was fixed with 10% formalin (12-24 hours at RT), incubated with HCN1 monoclonal antibody (Product # MA5-27589) using a dilution of 1:1000 (1 hour at RT), and followed by Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) and Mayer Hematoxylin (purple/blue) (30 minutes at RT) at a dilution of 250-500 µl.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HCN1 in paraffin-embedded human hippocampus. Sample was fixed with Bouin's Fixative Solution, incubated with HCN1 monoclonal antibody (Product # MA5-27589) using a dilution of 1:1000 (1 hour at RT), and followed by FITC Goat Anti-Mouse (green) (1 hour at RT) at a dilution of 1:50.
