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antibody from Invitrogen Antibodies
Targeting: KLRK1
CD314, D12S2489E, KLR, NKG2-D, NKG2D
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 14-5879-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD314 (NKG2D) Monoclonal Antibody (5C6), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 5C6 monoclonal antibody reacts with the human NKG2D, a 42 kDa lectin-like molecule expressed by NK cells, gammadelta T cells, some CD4^+ and CD8^+ T cells. Human NKG2D forms complexes with DAP10, a membrane adaptor protein, and has the ability to costimulate multiple NK activation receptors. The counter-receptor for human NKG2D has been identified as MICA/MICB expressed on epithelial tumors from lung, breast, kidney, ovary, prostate and colon carcinoma. 5C6 and 1D11 block binding of soluble MICA to gammadelta TCR T cell clones and inhibit lysis by these cells. 5C6 and 1D11 induced NKG2D function of redirected lysis of FcReceptor bearing P815 cells.
- Antibody clone number
- 5C6
- Concentration
- 0.5 mg/mL
Submitted references Heightened expression of the cytotoxicity receptor NKG2D correlates with acute and chronic nephropathy after kidney transplantation.
Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA.
Recognition of stress-induced MHC molecules by intestinal epithelial gammadelta T cells.
Seiler M, Brabcova I, Viklicky O, Hribova P, Rosenberger C, Pratschke J, Lodererova A, Matz M, Schönemann C, Reinke P, Volk HD, Kotsch K
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2007 Feb;7(2):423-33
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2007 Feb;7(2):423-33
Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA.
Bauer S, Groh V, Wu J, Steinle A, Phillips JH, Lanier LL, Spies T
Science (New York, N.Y.) 1999 Jul 30;285(5428):727-9
Science (New York, N.Y.) 1999 Jul 30;285(5428):727-9
Recognition of stress-induced MHC molecules by intestinal epithelial gammadelta T cells.
Groh V, Steinle A, Bauer S, Spies T
Science (New York, N.Y.) 1998 Mar 13;279(5357):1737-40
Science (New York, N.Y.) 1998 Mar 13;279(5357):1737-40
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- Invitrogen Antibodies (provider)
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- Experimental details
- Staining of normal human peripheral blood cells with 0.25 µg of Mouse IgG2a K Isotype Control Purified (Product # 14-4724-82) (open histogram) or 0.25 µg of Anti-Human CD314 (NKG2D) Purified (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85).Cells in the lymphocyte gate were used for analysis.
