Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
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Validation data
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- Product number
- PA5-18375 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- beta-Arrestin 2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of Human Brain lysate using Product # PA5-18375 at a concentration of 0.3 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of beta-Arrestin 2 by a beta-Arrestin 2 monoclonal antibody (Product # PA5-18375) at a concentration of 0.1 µg/mL. Human spleen lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-beta-Arrestin 2 Polyclonal Antibody (Product # PA5-18375) and a 28 kDa band corresponding to beta-Arrestin 2 was observed across cell lines tested. Whole cell extracts (30 µg lysate) of U-937 (Lane 1), HEL 92.1.7 (Lane 2), A549 (Lane 3), HEK-293 (Lane 4), MOLT-4 (Lane 5), SH-SY5Y (Lane 6), U-87 MG (Lane 7), Neuro-2a (Lane 8), HL-60 (Lane 9), tissue extract (30 µg lysate) of Mouse Brain (Lane 10) and Mouse Spleen (Lane 11) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence Rabbit anti-Goat IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).