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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [7]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-27662 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EEF2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, Neuro2A, NIH-3T3, Neuro 2A, Raw264.7, PC-12.
- Concentration
- 0.86 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EEF2 using A) 30 µg Neuro2A whole cell lysate and B) 30 µg NIH-3T3 whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an EEF2 polyclonal antibody (Product # PA5-27662) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of eEF2 in Various whole cell extracts (30 µg). Samples were separated by 7.5% SDS-PAGE and the membrane was probed with eEF2, Internal Polyclonal antibody (Product # PA5-27662) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using EEF2 Polyclonal Antibody (Product # PA5-27662). Sample (30 µg of whole cell lysate). A: 293T. Lane B: A431. 7.5% SDS PAGE. EEF2 Polyclonal Antibody (Product # PA5-27662) diluted at 1:1500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of EEF2 was performed by separating 30 µg of Whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a EEF2 Polyclonal Antibody (Product # PA5-27662) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using EEF2 Polyclonal Antibody (Product # PA5-27662). Sample (30 µg of whole cell lysate). A: 293T. Lane B: A431. 7.5% SDS PAGE. EEF2 Polyclonal Antibody (Product # PA5-27662) diluted at 1:1500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using EEF2 Polyclonal Antibody (Product # PA5-27662). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EEF2 Polyclonal Antibody (Product # PA5-27662) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of EEF2 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a EEF2 Polyclonal Antibody (Product # PA5-27662) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- eEF2 antibody detects eEF2 protein at cytosol on human colon by immunohistochemical analysis. Sample: Paraffin-embedded colon. EEF2 antibody (Product # PA5-27662) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- eEF2 antibody detects eEF2 protein at cytosol on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. EEF2 antibody (Product # PA5-27662) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
