Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- PA5-47373 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- K-cadherin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Cadherin-8, rhCadherin-11, and rhCadherin-12 is observed.
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of K-cadherin in immersion fixed MG‚63 human osteosarcoma cell line. Samples were incubated in K-cadherin polyclonal antibody (Product # PA5-47373) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MG-63 human osteosarcoma cell line was stained with Sheep Anti-human Cadherin-6/KCAD Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47373, filled histogram) or control antibodyopen histogram), followed by 557-conjugated Anti-Sheep IgG Secondary Antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of K-cadherin in MG‚63 human osteosarcoma cell line. Samples were incubated in K-cadherin polyclonal antibody (Product # PA5-47373) or control antibody followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody.