Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-21590 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PDE4D Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: A431, HeLa.
- Concentration
- 0.3 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PDE4D Polyclonal Antibody (Product # PA5-21590). Sample (30 µg of whole cell lysate). Lane A: A431. Lane B: HeLa. 7.5% SDS PAGE. PDE4D Polyclonal Antibody (Product # PA5-21590) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PDE4D in paraformaldehyde-fixed A431 cells using a PDE4D polyclonal antibody (Product # PA5-21590) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using PDE4D antibody (Product # PA5-21590) (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Product # MA1-25054) (Red) at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H441 xenograft, using PDE4D (Product # PA5-21590) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
