MAB10041-100
antibody from Novus Biologicals
Targeting: IL1RL1
DER4, FIT-1, IL33R, ST2, ST2L, ST2V, T1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB10041-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal ST2/IL-33R Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse ST2/IL-33 R in ELISAs.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of ST2/IL-33 R in P815 Mouse Cell Line by Flow Cytometry. P815 mouse mastocytoma cell line was stained with Rat Anti-Mouse ST2/IL-33 R Monoclonal Antibody (Catalog # MAB10041, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0113).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-33 and Neutralization by Mouse ST2/IL-33 R Antibody. Recombinant Human IL-33 induces proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by the Resazurin (Catalog # AR002). Under these conditions, proliferation elicited by IL-33 is neutralized (green line) by increasing concentrations of Rat Anti-Mouse ST2/IL-33 R Monoclonal Antibody (Catalog # MAB10041). The ND50 is typically 0.5-3 μg/mL.