BS8449

antibody from Bioworld Technology, Inc

Targeting: H4-16 HIST4H4, MGC24116

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Chromatin Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

BS8449

Provider

Bioworld Technology, Inc

Product name

Histone H4 (Mono Methyl K20) polyclonal antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide conjugated to KLH derived from within residues 1-100 of Human Histone H4, Mono-methylated at K20.

Description

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100ul

Concentration

1 mg/ml

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Bioworld Technology, Inc (provider)

Main image

Experimental details

Western blot analysis of extracts of HeLa cell line and H4 protein expressed in E.coli., using Histone H4 (Mono-Methyl K20) antibody.