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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Other assay [2]
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- Product number
- TA347209 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal H4K20me1 Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal H4K20me1 Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- HIST4H4
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- not determined
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB using the antibody against H4K20me1 diluted 1:750 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody against H4K20me1. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:8,000.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ChIP assays were performed using human U2OS cells. IgG (5 ug/IP) was used as negative control. qPCR primers were for the GAPDH promoter and for the coding region of MYOD, a gene that is inactive at normal conditions. Image shows the recovery, expressed as a % of input (the relative amount of IP'd DNA compared to input DNA after qPCR analysis).
- Validation comment
- Assay
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Dot Blot was performed with peptides containing other modifications of histone H4 or the unmodified sequence. Other histone modifications include mono- and dimethylation of the same lysine and acetylation of the nearby lysine 16. To determine the cross reactivity, 0.2 to 100 pmol of peptides were spotted on a membrane. Three different peptides for H4K16ac were used. The antibody was used at a dilution of 1:20,000. Image shows a high specificity of the antibody for the modification of interest.
- Validation comment
- DB
