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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Flow cytometry [2]
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Validation data
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- Product number
- TA502718 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502718, RRID:AB_11124989
- Product name
- C2orf40 mouse monoclonal antibody, clone OTI1A10 (formerly 1A10)
- Antibody type
- Monoclonal
- Description
- C2orf40 mouse monoclonal antibody, clone OTI1A10 (formerly 1A10)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ECRG4
- Isotype
- IgG
- Antibody clone number
- OTI1A10
- Vial size
- 100 µl
- Concentration
- 0.94 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C2orf40 (RC206239, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C2orf40.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-C2orf40 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC206239 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-C2orf40 antibody(TA502718), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-C2orf40 antibody(TA502718),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
