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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
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- Product number
- PA5-29192 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GTPBP4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, NIH-3T3, JC, BCL-1.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GTPBP4 Polyclonal Antibody (Product # PA5-29192). Sample (10 µg of whole cell lysate). Lane A: NIH-3T3. Lane B: JC. Lane C: BCL-1 . 7.5% SDS PAGE. GTPBP4 Polyclonal Antibody (Product # PA5-29192) diluted at 1:5,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GTPBP4 Polyclonal Antibody detects GTPBP4 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg A431 whole cell lysate/extract. C. 30 µg HeLa whole cell lysate/extract. D. 30 µg HepG2 whole cell lysate/extract.7.5 % SDS-PAGE. GTPBP4 Polyclonal Antibody (Product # PA5-29192) dilution: 1:5,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of GTPBP4 showing staining in the nucleolus of A431 cells. A431 cells were fixed in ice-cold MeOH for 5 min and stained using a GTPBP4 polyclonal antibody (Product # PA5-29192) diluted at 1:500. Blue: Hoechst 33343 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GTPBP4 Polyclonal Antibody detects GTPBP4 protein at nucleolus by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: GTPBP4 protein stained by GTPBP4 Polyclonal Antibody (Product # PA5-29192) diluted at 1:500. Blue: Hoechst 33343 staining.
