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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- MA5-24940 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Kindlin 2 Monoclonal Antibody (OTI25A4)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI25A4
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation was performed on HEK293T cells overexpressing FERMT2. Antigen-antibody complexes were formed by incubating the sample with 2 µg of FERMT2 monoclonal antibody (Product # MA5-24940) antibody and 0.1 mg of goat anti-mouse conjugated magnetic beads overnight. The sample was washed extensively and probed with rabbit anti-DDK polyclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FERMT2 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with FERMT2 (Product # MA5-24940) monoclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FERMT2 in HepG2, HeLa, HT29, A549, COS7, Jurkat, MDCK, PC12, MCF7 cells using 35 µg per lane. Samples were probed with FERMT2 (Product # MA5-24940) monoclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of FERMT2 was achieved by transfecting MDA-MB-231 with FERMT2 specific siRNA (Silencer® select Product # s21612, s21613). Western blot analysis (Fig. a) was performed using membrane enriched cell extracts from the FERMT2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Kindlin 2 Monoclonal Antibody (OTI25A4) (Product # MA5-24940, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to FERMT2.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Kindlin 2 Monoclonal Antibody (OTI25A4) (Product # MA5-24940) and a 78 kDa band corresponding to FERMT2, along with an uncharacterized band (*)was observed across all the cell lines tested. Membrane enriched extracts (30 µg lysate) of MDA-MB-231 (Lane 1), SK-BR-3 (Lane 2), BeWo (Lane 3), A549 (Lane 4) and SK-OV-3 (Lane 5) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of FERMT2 in HepG2 cells. Cells were probed with a FERMT2 monoclonal antibody (Product # MA5-24940).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human pancreas tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a FERMT2 monoclonal antibody (Product # MA5-24940).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human ovary tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a FERMT2 monoclonal antibody (Product # MA5-24940).
