702683

antibody from Invitrogen Antibodies

Targeting: ILK

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

702683

Provider

Invitrogen Antibodies

Product name

ILK Recombinant Rabbit Monoclonal Antibody (14H10L20)

Antibody type

Monoclonal

Antigen

Other

Description

This antibody is predicted to react with Monkey, Dog, Horse and Cat

Antibody clone number

14H10L20

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on Whole cell extracts (30 µg lysate) of HeLa (Lane 1), U-2 OS (Lane 2), U-87 MG (Lane 3), HEL 92.1.7 (Lane 4), K-562 (Lane 5) and HEK-293 (Lane 6). The blots were probed with Anti-Integrin-linked kinase (ILK) Recombinant Rabbit Monoclonal Antibody (Product # 702683, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 51 kDa band corresponding to Integrin-linked kinase was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex®NuPAGE®4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).