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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Flow cytometry [1]
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- Product number
- 17-5837-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD244 Monoclonal Antibody (eBioDM244), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBioDM244 monoclonal antibody reacts with human CD244 (2B4, p38). In human, CD244 is a 38 kDa protein expressed on NK cells, a subset of CD8+ T cells, gammadelta T cells, monocytes, basophils and eosinophils. Binding of the CD244 ligand, CD48, results in NK cell activation, unlike mouse CD244, which is an inhibitory receptor. Applications Reported: This eBioDM244 antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioDM244 antibody has been pre-titrated and tested by flow cytonetric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- eBioDM244
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references TIGIT expressing CD4+T cells represent a tumor-supportive T cell subset in chronic lymphocytic leukemia.
Cytomegalovirus-Induced Expression of CD244 after Liver Transplantation Is Associated with CD8+ T Cell Hyporesponsiveness to Alloantigen.
Molecular characterization of a novel human natural killer cell receptor homologous to mouse 2B4.
Catakovic K, Gassner FJ, Ratswohl C, Zaborsky N, Rebhandl S, Schubert M, Steiner M, Gutjahr JC, Pleyer L, Egle A, Hartmann TN, Greil R, Geisberger R
Oncoimmunology 2017;7(1):e1371399
Oncoimmunology 2017;7(1):e1371399
Cytomegalovirus-Induced Expression of CD244 after Liver Transplantation Is Associated with CD8+ T Cell Hyporesponsiveness to Alloantigen.
de Mare-Bredemeijer EL, Shi XL, Mancham S, van Gent R, van der Heide-Mulder M, de Boer R, Heemskerk MH, de Jonge J, van der Laan LJ, Metselaar HJ, Kwekkeboom J
Journal of immunology (Baltimore, Md. : 1950) 2015 Aug 15;195(4):1838-48
Journal of immunology (Baltimore, Md. : 1950) 2015 Aug 15;195(4):1838-48
Molecular characterization of a novel human natural killer cell receptor homologous to mouse 2B4.
Boles KS, Nakajima H, Colonna M, Chuang SS, Stepp SE, Bennett M, Kumar V, Mathew PA
Tissue antigens 1999 Jul;54(1):27-34
Tissue antigens 1999 Jul;54(1):27-34
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD56 (NCAM) PE (Product # 12-0567-42) and Mouse IgG1 kappa Isotype Control APC (Product # 17-4714-81) (left) or Anti-Human CD244 APC (right). Cells in the lymphocyte gate were used for analysis.
