MAB7329

antibody from Novus Biologicals

Targeting: VAPB ALS8, VAP-B, VAP-C

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB7329

Provider

Novus Biologicals

Product name

Mouse Monoclonal VAP-B Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects rat VAP-B in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) VAP-B and approximately 50% cross-reactivity with rhVAP-A is observed.

Reactivity

Rat

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Rat VAP-B by Western Blot. Western blot shows lysates of PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Rat VAP-B Monoclonal Antibody (Catalog # MAB7329) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VAP-B at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

VAP-B in Rat Brain. VAP-B was detected in perfusion fixed frozen sections of rat brain (medulla) using Mouse Anti-Rat VAP-B Monoclonal Antibody (Catalog # MAB7329) at 8 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to gigantocellular neurons. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.