Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-81112 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- VAPB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of VAPB in Lane A: HepG2 Whole Cell Lysate (60 µg), Lane B: A549 Whole Cell Lysate (60 µg) . Samples were probed using a VAPB Polyclonal Antibody (Product # PA5-81112) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:27 kDa. Observed band size:30 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using VAPB Polyclonal Antibody (Product # PA5-81112) at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate, Lane B: A549 Whole Cell Lysate. Lysates/proteins at 60 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 27 kDa. Observed band size: 30 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human VAPB in human liver with VAPB Polyclonal Antibody (Product # PA5-81112, 1:1,000, formalin-fixed paraffin embedded sections).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- VAPB Immunoprecipitation using: Lane A: 0.5 mg HepG2 Whole Cell Lysate, Lane B: 0.5 mg Jurkat Whole Cell Lysate, Lane C: 0.5 mg A549 Whole Cell Lysate 2 µL with VAPB Polyclonal Antibody (Product # PA5-81112) and 15 µL of 50 % Protein G agarose. Primary antibody: VAPB Polyclonal Antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 27 kDa. Observed band size: 27 kDa.