AF4848

antibody from Novus Biologicals

Targeting: CRYAA CRYA1, HSPB4

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF4848

Provider

Novus Biologicals

Product name

Goat Polyclonal AlphaA Crystallin/CRYAA Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects mouse AlphaA Crystallin/CRYAA in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human CRYAB is observed.

Reactivity

Human, Mouse

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human/Mouse AlphaA Crystallin/CRYAA by Western Blot. Western blot shows lysates of mouse eye lens tissue. PVDF membrane was probed with 0.1 µg/mL Goat Anti-Human/Mouse AlphaA Crystallin/CRYAA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4848) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human CRYAA and CRYAB (5 ng/lane) were included. Bands for AlphaA Crystallin/CRYAA were detected at approximately 20 - 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Mouse AlphaA Crystallin/CRYAA by Simple Western^TM. Simple Western lane view shows lysates of mouse eye lens tissue, loaded at 0.2 mg/mL. A specific band was detected for AlphaA Crystallin/CRYAA at approximately 28 kDa (as indicated) using 1 µg/mL of Goat Anti-Human/Mouse AlphaA Crystallin/CRYAA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4848) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.