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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
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- Product number
- PA3-304 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-GRPR Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Several different molecular weight isoforms of CXCR4 have been described in the literature, which vary depending on cell type tested and extraction buffer used. Using Ramos, Raji, and Jurkat membrane fractions, two different MW forms of CXCR4 were detected with PA3-305 by Western blot, between 55-72kD. It is recommended to use a membrane isolation buffer/kit for extracting CXCR4 for Western blot analysis. Cellular membrane fractions were isolated for Western blot using Mem-PER Plus (Product # 89842).
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- Lot Dependent
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Immunohistochemical detection of bombesin receptor subtypes GRP-R and BRS-3 in human tumors using novel antipeptide antibodies.
Schulz S, Röcken C, Schulz S
Virchows Archiv : an international journal of pathology 2006 Oct;449(4):421-7
Virchows Archiv : an international journal of pathology 2006 Oct;449(4):421-7
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of GRPR in paraffin embedded human mammary carcinoma tissue sections using Product # PA3-304.
