TA501536

antibody from OriGene

Targeting: BTN1A1 BT, BTN, BTN1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA501536

Provider

OriGene

Proper citation

OriGene Cat#TA501536, RRID:AB_11126853

Product name

Anti-BTN1A1 mouse monoclonal antibody, clone OTI5E6 (formerly 5E6)

Antibody type

Monoclonal

Description

Anti-BTN1A1 mouse monoclonal antibody, clone OTI5E6 (formerly 5E6)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

BTN1A1

Isotype

IgG

Antibody clone number

OTI5E6

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTN1A1 (RC223852, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTN1A1.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BTN1A1 monoclonal antibody.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-BTN1A1 mouse monoclonal antibody (TA501536) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTN1A1(RC223852).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC223852 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-BTN1A1 antibody(TA501536), and then analyzed by flow cytometry.

Validation comment

FC