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- Western blot [1]
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- Product number
- PA5-42729 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRAP alpha Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: DDEAEVEEDE PTDLVEDKEE EDVSGEPEAS PSADTTILFV KGEDFPANNI
- Concentration
- 0.5 mg/mL
Submitted references Fermented Sea Tangle (Laminaria japonica Aresch) Suppresses RANKL-Induced Osteoclastogenesis by Scavenging ROS in RAW 264.7 Cells.
Jeong JW, Ji SY, Lee H, Hong SH, Kim GY, Park C, Lee BJ, Park EK, Hyun JW, Jeon YJ, Choi YH
Foods (Basel, Switzerland) 2019 Jul 26;8(8)
Foods (Basel, Switzerland) 2019 Jul 26;8(8)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human HepG2 cell lysate using an anti-SSR1 polyclonal antibody (Product # PA5-42729).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of human kidney tissue using an anti-SSR1 polyclonal antibody (Product # PA5-42729).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Inhibition of RANKL-stimulated osteoclast differentiation by FST in RAW 264.7 mouse macrophage-like cells. Cells were stimulated with 100 ng/mL RANKL in the presence or absence of FST or 100 ng/mL OPG for 5 days. ( A ) Representative photographs of the morphological changes are presented. ( B ) Cells were fixed and stained for TRAP and examined under an inverted microscope. ( C ) TRAP-positive multinucleated cells were counted to determine osteoclast numbers. ( D ) Supernatants were collected from cells grown under the same conditions and TRAP activities were measured using an ELISA reader. Results are presented as means +- SD of three independent experiments. * p < 0.05, ** p < 0.01 and *** p < 0.001 indicates significant difference compared to RANKL-treated cells. RANKL: receptor of activator of nuclear factor kappa-B ligand; FST: fermented sea tangle extract; OPG: osteoprotegerin; TRAP: tartrate-resistance acid phosphatase; +: cells treated the reagent; -: cells untreated the reagent.
