TA808771

antibody from OriGene

Targeting: CD274 B7-H, B7-H1, B7H1, PD-L1, PDCD1LG1, PDL1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA808771

Provider

OriGene

Proper citation

OriGene Cat#TA808771, RRID:AB_2628988

Product name

CD274 mouse monoclonal antibody, clone OTI9E12 (formerly 9E12)

Antibody type

Monoclonal

Description

CD274 mouse monoclonal antibody, clone OTI9E12 (formerly 9E12)

Host

Mouse

Conjugate

Unconjugated

Epitope

CD274

Isotype

IgG

Antibody clone number

OTI9E12

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD274 (RC213071, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD274.(1:500)

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunocytochemistry staining of stable expression CD274 cells using anti-CD274 mouse monoclonal antibody (TA808771)(Left). The right is negative control.(1:5000)(1:2000)

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of stable expression CD274(RC213071) in HEK293T cells (Red) compared to negative control HEK293T cells (Blue) using anti-CD274 antibody(TA808771).(1:100)

Validation comment

FC