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antibody from Invitrogen Antibodies
Targeting: DENND5A
FLJ22354, FLJ33829, FLJ43455, KIAA1091, RAB6IP1
Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- 702789 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DENND5A Recombinant Rabbit Monoclonal Antibody (14H6L8)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with Monkey, Mouse, Rat.
- Antibody clone number
- 14H6L8
- Concentration
- 0.5 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on Membrane enriched extracts (30µg lysate) of IMR-32 (Lane 1), SH-SY5Y (Lane 2), SK-N-AS (Lane 3), PC12 (Lane 4) and tissue extracts of Mouse Brain (Lane 5) and Rat Brain (Lane6). The blots were probed with Anti-DENND5A Recombinant Rabbit Monoclonal Antibody (Product # 702789, 1:5,000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4,000 dilution). A 160 kDa band corresponding to DENND5A was observed across the cell lines and tissues tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on Membrane enriched extracts (30µg lysate) of IMR-32 (Lane 1), SH-SY5Y (Lane 2), SK-N-AS (Lane 3), PC12 (Lane 4) and tissue extracts of Mouse Brain (Lane 5) and Rat Brain (Lane6). The blots were probed with Anti-DENND5A Recombinant Rabbit Monoclonal Antibody (Product # 702789, 1:5,000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4,000 dilution). A 160 kDa band corresponding to DENND5A was observed across the cell lines and tissues tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of DENND5A was achieved by transfecting HeLa cells with DENND5A specific siRNA (Silencer® select Product # s23415 & s23414). Western blot analysis (Fig a) Was performed using Membrane enriched extracts from DENND5A knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blots were probed with Anti-DENND5A Recombinant Rabbit Monoclonal Antibody (Product # 702789, 1:5,000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4,000 dilution). Densitometric analysis of this Western blot is shown in histogram (Fig b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to DENND5A.
