NBP2-36571

antibody from Novus Biologicals

Targeting: CANX CNX, IP90, P90

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-36571

Provider

Novus Biologicals

Product name

Mouse Monoclonal Calnexin Antibody

Antibody type

Monoclonal

Description

Protein G purified.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

1.0 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Exosomal miR-16-5p as a target for malignant mesothelioma.

Munson PB, Hall EM, Farina NH, Pass HI, Shukla A
Scientific reports 2019 Aug 12;9(1):11688

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Actin polymerization plays a significant role in asbestos-induced inflammasome activation in mesothelial cells in vitro.

MacPherson M, Westbom C, Kogan H, Shukla A
Histochemistry and cell biology 2017 May;147(5):595-604

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Calnexin Antibody (IE2.1C12) [NBP2-36571] - Analysis of 11 kDa Partial Recombinant Human Calnexin protein with Calnexin antibody (clone IE2.1C12) at 0.5 ug/ml concentration.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) [NBP2-36571] - Analysis of FFPE tissue section of malignant stromal tumor of the human small bowel using mouse monoclonal Calnexin antibody (clone IE2.1C12) at 7 ug/ml concentration. The cancer cells showed a very strong cytoplasmic reactivity for Calnexin.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) [NBP2-36571] - Analysis of FFPE tissue section of human skin using mouse monoclonal Calnexin antibody (clone IE2.1C12) at 7 ug/ml concentration. The outermost keratinocytes layer of the epidermis showed cytoplasmic positivity for Calnexin protein.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) [NBP2-36571] - Analysis of FFPE tissue section of normal human breast using mouse monoclonal Calnexin antibody (clone IE2.1C12) at 7 ug/ml concentration. The myoepithelial cells around the lobules depicted a very strong cytoplasmic staining.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) [NBP2-36571] - Analysis of FFPE tissue section of normal human kidney using mouse monoclonal Calnexin antibody (clone IE2.1C12) at 7 ug/ml concentration. The cells of Glomeruli developed strong cytoplasmic staining.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Calnexin Antibody (IE2.1C12) [NBP2-36571] - Analysis of FFPE tissue section of human placenta using mouse monoclonal Calnexin antibody (clone IE2.1C12) at 7 ug/ml concentration.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow (Intracellular): Calnexin Antibody (IE2.1C12) [NBP2-36571] - Intracellular staining of human Calnexin in Flow cytometry using 5.0 ug of antibody per 1 million cells. Isotype control was mouse IgG2b kappa.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Calnexin Antibody (IE2.1C12) [NBP2-36571] - Analysis of Allophycocyanin conjugate of NBP2-36571. An intracellular stain was performed on Jurkat cells with Calnexin antibody (IE2.1C12) NBP2-36571APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin.